The cytokine protein Interleukin-1β is a mediator in the inflammatory response and is a therapeutic target for autoinflammatory diseases such as Alzheimer’s disease and Schnitzler syndrome. The IL-1β (Human) Colorimetric Detection Kit is designed for detecting and quantifying human interleukin-1β in cell culture medium. Only a few simple steps on a microtiter plate are required for the assay. First, the capture antibody is coated on a 96-well plate. Next, samples containing IL-1β are incubated on the coated plate followed by detecting the captured IL-1 β with the detection antibody. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.